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Anti-Mouse IL-5 R alpha/CD125 Polyclonal Antibody (MX243014)

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Anti-Mouse IL-5 R alpha/CD125 Polyclonal Antibody
Anti-Mouse IL-5 R alpha/CD125 Polyclonal Antibody
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Vista general
Número de catálogoMX243014
Descripción
Anti-Mouse CD125/IL5RA Polyclonal Antibody (MX243014) is a rabbit polyclonal antibody detecting IL5RA in ELISA, IHC, WB. Suitable for Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesMouse
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Mouse CD125/IL5RA (Asn32-Gly329).
Objetivo IL-5R subunit alpha, IL-5 receptor subunit alpha, IL5RA, IL-5RA, IL-5R-alpha, Interleukin-5 receptor subunit alpha, CDw125, CD125, IL5R
Purificación Purified by antigen affinity column.
Número de acceso P21183
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Interleukin-5 receptor subunit alpha (CD125/IL5RA) is a ~46 kDa protein. Cell surface receptor that plays an important role in the survival, differentiation, and chemotaxis of eosinophils. Acts by forming a heterodimeric receptor with CSF2RB subunit and subsequently binding to interleukin-5. In unstimulated conditions, interacts constitutively with JAK2. Heterodimeric receptor activation leads to JAK2 stimulation and subsequent activation of the JAK-STAT pathway.

1. Barry, SC. et al. (1991) Cytokine 3, 339-44. PMID: 1873482
Nota For research use only.
Imágenes
  • Anti-Mouse IL-5 R alpha/CD125 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-IL5RA polyclonal antibody at 1ug/mL on various samples.
    Lane 1: HL-60 cell lysate
    Lane 2: Mouse lung lysate

Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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