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| カタログ番号 | MC336207 |
|---|---|
| 種反応性 | Mouse |
| アプリケーション | in vivo 4-1BB stimulation, in vitro 4-1BB stimulation |
| 宿主種 | Rat |
| クローン性 | Monoclonal |
| アイソタイプ | IgG2a, kappa |
| クローンID | 3H3 |
| テスト済みアプリケーション | FCM |
| ターゲット | 4-1BB ligand receptor, CD137, CDw137, ILA, T-cell antigen 4-1BB homolog, T-cell antigen ILA, TNFRSF9, Tumor necrosis factor receptor superfamily member 9 |
| エンドトキシンレベル | < 10 EU/mg |
| 純度 | >95% as determined by SDS-PAGE. |
| 精製 | Protein A/G purified from cell culture supernatant. |
| アクセッション番号 | P20334 |
| 形態 | Liquid |
| 保存バッファー | 0.01M PBS pH 7.4. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 背景 | Tumor necrosis factor receptor superfamily member 9 (CD137/4-1BB) is a ~27 kDa protein. Receptor for TNFSF9/4-1BBL. Conveys a signal that enhances CD8(+) T-cell survival, cytotoxicity, and mitochondrial activity, thereby promoting immunity against viruses and tumors. |
| 注意事項 | For research use only. |
Detects CD137/4-1BB/TNFRSF9 in indirect ELISAs.
Flow-cytometry using anti-mouse CD137 antibody.CD137/4-1BB/TNFRSF9 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-mouse CD137 antibody monoclonal antibody (Catalog # MC336207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-rat antibody and cells analysed on a NovoCyte Flow Cytometer.
Immunofluorescence staining of HDLM2 with Rat monoclonal antibody (20ug/ml, overnight at 4°C), followed by Alexa Fluor-488-conjugated secondary antibody (6.5ug/ml, 50minutes at room temperature). The target protein was detected in the cell membrane.
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