Anti-Mouse CD40 Ligand/TNFSF5 Recombinant Antibody (MR1/MR-1) (MB199107)

Name: Anti-Mouse CD40 Ligand/TNFSF5 Recombinant Antibody (MR1/MR-1)
Brand: abinScience
SKU: MB199107
Price: 191 USD
Availability: InStock

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Aperçu

Numéro de catalogue	MB199107
Reactivité des espèces	Mouse
Applications	Blocking, FCM, IHC
Espèce hôte	Rat
Clonalité	Monoclonal
Isotype	IgG1, kappa
ID de clone	MR1/MR-1
Applications testées	FCM
Cible	CD154, CD40 ligand, CD40 ligand, membrane form, CD40 ligand, soluble form, CD40-L, CD40L, CD40LG, T-cell antigen Gp39, TNF-related activation protein, TNFSF5, TRAP, Tumor necrosis factor ligand superfamily member 5, sCD40L
Niveau d'endotoxines	< 10 EU/mg
Pureté	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Numéro d'accès	P27548
Forme	Liquid
Tampon de stockage	0.01M PBS pH 7.4. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte	CD40 ligand (CD154/CD40L) is a ~29 kDa protein. Cytokine that acts as a ligand to CD40/TNFRSF5. Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation. Induces the activation of NF-kappa-B. Induces the activation of kinases MAPK8 and PAK2 in T-cells. 1. Armitage, RJ. et al. (1992) Nature 357, 80-2. PMID: 1374165
Note	For research use only.

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Flow Cytometry

Flow-cytometry using anti-mouse CD154 antibody.CD154 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-mouse CD154 antibody monoclonal antibody (Catalog # MB199107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-rat antibody and cells analysed on a NovoCyte Flow Cytometer.

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Fiche technique

Anti-Mouse CD40 Ligand/TNFSF5 Recombinant Antibody (MR1/MR-1).pdf

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