中文
English
한국어
日本語
Español
Français
Русский
| カタログ番号 | MB199107 |
|---|---|
| 種反応性 | Mouse |
| アプリケーション | Blocking, FCM, IHC |
| 宿主種 | Rat |
| クローン性 | Monoclonal |
| アイソタイプ | IgG1, kappa |
| クローンID | MR1/MR-1 |
| テスト済みアプリケーション | FCM |
| ターゲット | CD154, CD40 ligand, CD40 ligand, membrane form, CD40 ligand, soluble form, CD40-L, CD40L, CD40LG, T-cell antigen Gp39, TNF-related activation protein, TNFSF5, TRAP, Tumor necrosis factor ligand superfamily member 5, sCD40L |
| エンドトキシンレベル | < 10 EU/mg |
| 純度 | >95% as determined by SDS-PAGE. |
| 精製 | Protein A/G purified from cell culture supernatant. |
| アクセッション番号 | P27548 |
| 形態 | Liquid |
| 保存バッファー | 0.01M PBS pH 7.4. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 背景 | CD40 ligand (CD154/CD40L) is a ~29 kDa protein. Cytokine that acts as a ligand to CD40/TNFRSF5. Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation. Induces the activation of NF-kappa-B. Induces the activation of kinases MAPK8 and PAK2 in T-cells. 1. Armitage, RJ. et al. (1992) Nature 357, 80-2. PMID: 1374165 |
| 注意事項 | For research use only. |
Flow-cytometry using anti-mouse CD154 antibody.CD154 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-mouse CD154 antibody monoclonal antibody (Catalog # MB199107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-rat antibody and cells analysed on a NovoCyte Flow Cytometer.
+86-027-65523339
中国武漢市深敦寺路666号C棟、武漢、430206
