Please ensure Javascript is enabled for purposes of website accessibility
Главная > Продукты > Антитела > Антитела для Цитометрии Протока

Anti-Mouse CD40 Ligand/TNFSF5 Recombinant Antibody (MR1/MR-1) (MB199107)

Anti-Mouse CD40 Ligand/TNFSF5 Recombinant Antibody (MR1/MR-1)
Anti-Mouse CD40 Ligand/TNFSF5 Recombinant Antibody (MR1/MR-1)
Цена(USD):
Спецификация:
  • 50ug
  • 100ug
Количество:
Свяжитесь с нами
  • Обзор

  • Изображения

  • Ссылки

  • Даташит

  • SDS

Обзор
Каталожный номерMB199107
Реактивность видовMouse
ПримененияBlocking, FCM, IHC
Хозяинский видRat
КлоональностьMonoclonal
ИзотипIgG1, kappa
ID клонаMR1/MR-1
Проверенные приложения FCM
Цель CD154, CD40 ligand, CD40 ligand, membrane form, CD40 ligand, soluble form, CD40-L, CD40L, CD40LG, T-cell antigen Gp39, TNF-related activation protein, TNFSF5, TRAP, Tumor necrosis factor ligand superfamily member 5, sCD40L
Уровень эндотоксинов < 10 EU/mg
Чистота >95% as determined by SDS-PAGE.
Очистка Protein A/G purified from cell culture supernatant.
Номер доступа P27548
Форма Liquid
Буфер хранения 0.01M PBS pH 7.4.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

CD40 ligand (CD154/CD40L) is a ~29 kDa protein. Cytokine that acts as a ligand to CD40/TNFRSF5. Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation. Induces the activation of NF-kappa-B. Induces the activation of kinases MAPK8 and PAK2 in T-cells.

1. Armitage, RJ. et al. (1992) Nature 357, 80-2. PMID: 1374165
Примечание For research use only.
Изображения
  • Anti-Mouse CD40 Ligand/TNFSF5 Recombinant Antibody (MR1/MR-1)

    Flow Cytometry

    Flow-cytometry using anti-mouse CD154 antibody.CD154 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-mouse CD154 antibody monoclonal antibody (Catalog # MB199107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-rat antibody and cells analysed on a NovoCyte Flow Cytometer.

Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Рекомендация

Свяжитесь с нами для индивидуальных предложений, оптовых запросов и других вопросов.

Почта: [email protected]

Список дистрибьюторов