

| Каталожный номер | MB199107 |
|---|---|
| Реактивность видов | Mouse |
| Применения | Blocking, FCM, IHC |
| Хозяинский вид | Rat |
| Клоональность | Monoclonal |
| Изотип | IgG1, kappa |
| ID клона | MR1/MR-1 |
| Проверенные приложения | FCM |
| Цель | CD154, CD40 ligand, CD40 ligand, membrane form, CD40 ligand, soluble form, CD40-L, CD40L, CD40LG, T-cell antigen Gp39, TNF-related activation protein, TNFSF5, TRAP, Tumor necrosis factor ligand superfamily member 5, sCD40L |
| Уровень эндотоксинов | < 10 EU/mg |
| Чистота | >95% as determined by SDS-PAGE. |
| Очистка | Protein A/G purified from cell culture supernatant. |
| Номер доступа | P27548 |
| Форма | Liquid |
| Буфер хранения | 0.01M PBS pH 7.4. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии. |
| Устойчивость и хранение | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Фон | CD40 ligand (CD154/CD40L) is a ~29 kDa protein. Cytokine that acts as a ligand to CD40/TNFRSF5. Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation. Induces the activation of NF-kappa-B. Induces the activation of kinases MAPK8 and PAK2 in T-cells. 1. Armitage, RJ. et al. (1992) Nature 357, 80-2. PMID: 1374165 |
| Примечание | For research use only. |

Flow-cytometry using anti-mouse CD154 antibody.CD154 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-mouse CD154 antibody monoclonal antibody (Catalog # MB199107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-rat antibody and cells analysed on a NovoCyte Flow Cytometer.




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