Anti-Mouse CD20 Recombinant Antibody (18B12) (MY257107)

Name: Anti-Mouse CD20 Recombinant Antibody (18B12)
Brand: abinScience
SKU: MY257107
Price: 196 USD
Availability: InStock

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Aperçu

Numéro de catalogue	MY257107
Reactivité des espèces	Mouse
Applications	FCM, IF, FuncS, WB
Espèce hôte	Mouse
Clonalité	Monoclonal
Isotype	IgG2a, kappa
ID de clone	18B12
Applications testées	FCM
Cible	B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, CD20, Leukocyte surface antigen Leu-16, MS4A1, Membrane-spanning 4-domains subfamily A member 1
Niveau d'endotoxines	< 10 EU/mg
Pureté	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Numéro d'accès	P19437
Forme	Liquid
Tampon de stockage	0.01M PBS pH 7.4. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte	B-lymphocyte antigen CD20 (MS4A1) is a ~31 kDa protein. B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes. Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR. CD20 is the therapeutic target of rituximab (MabThera) and obinutuzumab (Gazyva).
Note	For research use only.

Images

Flow Cytometry

Flow-cytometry using anti-Mouse CD20 antibody.CD20 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-Mouse CD20 antibody monoclonal antibody (Catalog # MY257107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Anti-Mouse CD20/MS4A1 Antibody (18B12)

Reférences

Fiche technique

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Aperçu