Anti-Mouse CD20 Recombinant Antibody (MB20-11) (MY257013)

Name: Anti-Mouse CD20 Recombinant Antibody (MB20-11)
Brand: abinScience
SKU: MY257013
Price: 191 USD
Availability: InStock

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Spécification :

50ug
100ug
1mg

Nombre :

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

MY257013

Reactivité des espèces

Mouse

Applications

ELISA, FCM, Functional assay, Research in vivo

Espèce hôte

Mouse

Clonalité

Monoclonal

Isotype

IgG2c, kappa

ID de clone

MB20-11

Applications testées

FCM

Cible

B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, CD20, Leukocyte surface antigen Leu-16, MS4A1, Membrane-spanning 4-domains subfamily A member 1

Niveau d'endotoxines

< 10 EU/mg

Pureté

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Numéro d'accès

P19437

Forme

Liquid

Tampon de stockage

0.01M PBS pH 7.4.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:2000-1:20000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

B-lymphocyte antigen CD20 (MS4A1) is a ~31 kDa protein. B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes. Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR. CD20 is the therapeutic target of rituximab (MabThera) and obinutuzumab (Gazyva).

Note

For research use only

Images

Flow cytometry

Flow-cytometry using FITC anti-Mouse CD20 antibody. Mouse spleen cells were stained with an irrelevant antibody (Blue Histogram) or an FITC conjugated anti-Mouse CD20 monoclonal antibody (Catalog MY257013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing,cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry using anti-Mouse CD20 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-Mouse CD20 monoclonal antibody (Catalog MY257013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience:MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Reférences

Fiche technique

Anti-Mouse CD20 Recombinant Antibody (MB20-11).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

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