Anti-Mouse CD20 Recombinant Antibody (MB20-11) (MY257013)

Name: Anti-Mouse CD20 Recombinant Antibody (MB20-11)
Brand: abinScience
SKU: MY257013
Price: 191 USD
Availability: InStock

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Спецификация:

50ug
100ug
1mg

Количество:

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Обзор
Изображения
Ссылки
Даташит
SDS

Обзор

Каталожный номер

MY257013

Реактивность видов

Mouse

Применения

ELISA, FCM, Functional assay, Research in vivo

Хозяинский вид

Mouse

Клоональность

Monoclonal

Изотип

IgG2c, kappa

ID клона

MB20-11

Проверенные приложения

FCM

Цель

B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, CD20, Leukocyte surface antigen Leu-16, MS4A1, Membrane-spanning 4-domains subfamily A member 1

Уровень эндотоксинов

< 10 EU/mg

Чистота

>95% as determined by SDS-PAGE.

Очистка

Protein A/G purified from cell culture supernatant.

Номер доступа

P19437

Форма

Liquid

Буфер хранения

0.01M PBS pH 7.4.

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Информация об использовании продукта

Применение	Разбавление
ELISA	1:2000-1:20000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

B-lymphocyte antigen CD20 (MS4A1) is a ~31 kDa protein. B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes. Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR. CD20 is the therapeutic target of rituximab (MabThera) and obinutuzumab (Gazyva).

Примечание

For research use only

Изображения

Flow cytometry

Flow-cytometry using FITC anti-Mouse CD20 antibody. Mouse spleen cells were stained with an irrelevant antibody (Blue Histogram) or an FITC conjugated anti-Mouse CD20 monoclonal antibody (Catalog MY257013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing,cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry using anti-Mouse CD20 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-Mouse CD20 monoclonal antibody (Catalog MY257013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience:MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Ссылки

Даташит

Anti-Mouse CD20 Recombinant Antibody (MB20-11).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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