Anti-Mouse Endosialin/CD248 Recombinant Antibody (G78) (MV681107)

Name: Anti-Mouse Endosialin/CD248 Recombinant Antibody (G78)
Brand: abinScience
SKU: MV681107
Price: 191 USD
Availability: InStock

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

MV681107

Reactivité des espèces

Human, Mouse

Applications

ELISA, FCM, in vivo study

Espèce hôte

Human

Clonalité

Monoclonal

Isotype

IgG1, lambda

ID de clone

G78

Applications testées

FCM

Cible

Endosialin, TEM1, CD164L1, Tumor endothelial marker 1, CD248

Niveau d'endotoxines

Please contact with the lab for this information.

Pureté

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Numéro d'accès

Q91V98

Forme

Liquid

Tampon de stockage

0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Reconstitution

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:2000-1:20000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Contexte

Endosialin (CD248) is a ~81 kDa protein. Cell surface glycoprotein involved in various biological processes including angiogenesis, immune response modulation, and tissue remodeling and repair. Participates in pericyte proliferation through positive modulation of the PDGF receptor signaling pathway. Acts as a scaffold for factor X, triggering allosteric changes and the spatial re-alignment of factor X with the TF-factor VIIa complex, thereby enhancing coagulation activation. Modulates the insulin signaling pathway by interacting with insulin receptor/INSR and by diminishing its capacity to be autophosphorylated in response to insulin. Also regulates LPS-induced inflammatory responses in macrophages by favoring production of proinflammatory cytokines.

1. Opavsky, R. et al. (2001) The Journal of biological chemistry 276, 38795-807. PMID: 11489895

2. Naylor, AJ. et al. (2014) PloS one 9, e107146. PMID: 25243742

3. Benedet, PO. et al. (2024) EBioMedicine 99, 104906. PMID: 38061240

4. Hong, YK. et al. (2019) The Journal of investigative dermatology 139, 2204-2214.e7. PMID: 30986375

5. Kapopara, PR. et al. (2021) Journal of thrombosis and haemostasis : JTH 19, 1932-1947. PMID: 33830628

6. Cheng, TL. et al. (2021) Translational research : the journal of laboratory and clinical medicine 232, 150-162. PMID: 33737161

Note

For research use only.

Images

Flow Cytometry

Flow-cytometry using anti-mouse CD248 antibody.CD248 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-mouse CD248 antibody monoclonal antibody (Catalog # MV681107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Anti-Mouse CD248 Antibody (G78)

Reférences

Fiche technique

Anti-Mouse Endosialin/CD248 Recombinant Antibody (G78).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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