Anti-Mouse CD25/IL2R alpha Recombinant Antibody (PC61/PC-61.5.3), FITC (MF996117)

Name: Anti-Mouse CD25/IL2R alpha Recombinant Antibody (PC61/PC-61.5.3), FITC
Brand: abinScience
SKU: MF996117
Price: 118 USD
Availability: InStock

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Aperçu

Numéro de catalogue	MF996117
Reactivité des espèces	Mouse
Applications	FCM
Espèce hôte	Rat
Clonalité	Monoclonal
Isotype	IgG1, lambda
ID de clone	PC61/PC-61.5.3
Conjugaison	FITC
Applications testées	FCM
Cible	CD25, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, IL2RA, Interleukin-2 receptor subunit alpha, TAC antigen, p55
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Produits associés	Rat IgG1 Recombinant Isotype Control Antibody (HyHEL-10), FITC
Purification	Protein A/G purified from cell culture supernatant.
Numéro d'accès	P01590
Forme	Liquid
Tampon de stockage	0.01M PBS, pH 7.4, 50% glycerol, 0.05% Proclin 300. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Stabilité et stockage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Contexte	Interleukin-2 receptor subunit alpha (CD25/IL2RA) is a ~30 kDa protein. Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

Images

Flow CytoMetry

Flow-cytometry using FITC anti-Mouse CD25 antibody. Unstimulated cells (blue Histogram) and 3-day CoA-stimulated Mouse spleen cells (Yellow Histogram) were stained with an FITC anti-Mouse CD25 monoclonal antibody (Catalog: MF996117) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Reférences

Fiche technique

Anti-Mouse CD25/IL2R alpha Recombinant Antibody (PC61/PC-61.5.3), FITC.pdf

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