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Anti-Mouse CD25/IL2R alpha Recombinant Antibody (PC61/PC-61.5.3), FITC (MF996117)

Anti-Mouse CD25/IL2R alpha Recombinant Antibody (PC61/PC-61.5.3), FITC
Anti-Mouse CD25/IL2R alpha Recombinant Antibody (PC61/PC-61.5.3), FITC
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Спецификация:
  • 50T
  • 100T
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Обзор
Каталожный номерMF996117
Реактивность видовMouse
ПримененияFCM
Хозяинский видRat
КлоональностьMonoclonal
ИзотипIgG1, lambda
ID клонаPC61/PC-61.5.3
КонъюгацияFITC
Проверенные приложения FCM
Цель CD25, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, IL2RA, Interleukin-2 receptor subunit alpha, TAC antigen, p55
Уровень эндотоксинов Please contact with the lab for this information.
Чистота >90% as determined by SDS-PAGE.
Связанные продукты Rat IgG1 Recombinant Isotype Control Antibody (HyHEL-10), FITC
Очистка Protein A/G purified from cell culture supernatant.
Номер доступа P01590
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Устойчивость и хранение Store at 4°C for 12 months. Protect from light. Do not freeze.
Фон

Interleukin-2 receptor subunit alpha (CD25/IL2RA) is a ~30 kDa protein. Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.

Примечание For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
Изображения
  • Anti-Mouse CD25/IL2R alpha Recombinant Antibody (PC61/PC-61.5.3), FITC

    Flow CytoMetry

    Flow-cytometry using FITC anti-Mouse CD25 antibody. Unstimulated cells (blue Histogram) and 3-day CoA-stimulated Mouse spleen cells (Yellow Histogram) were stained with an FITC anti-Mouse CD25 monoclonal antibody (Catalog: MF996117) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Ссылки
Formula
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