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Anti-Mouse CD321/F11R Polyclonal Antibody
Anti-Mouse CD321/F11R Polyclonal Antibody
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Спецификация:
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Обзор
Каталожный номерMV592014
Описание
Anti-Mouse CD321/F11R Polyclonal Antibody (MV592014) is a rabbit polyclonal antibody detecting CD321/F11R in ELISA, IHC, WB. Suitable for Mouse, Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Реактивность видовMouse, Rat
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Mouse CD321/F11R (Gly53-Glu233).
Цель Junctional adhesion molecule A (JAM-A) (Junctional adhesion molecule 1) (JAM-1) (CD antigen CD321)
Уровень эндотоксинов Please contact with the lab for this information.
Чистота >95% as determined by SDS-PAGE.
Очистка Purified by antigen affinity column.
Номер доступа O88792
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Junctional adhesion molecule A (CD321/F11R) is a ~32 kDa protein. Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly. Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Ligand for integrin alpha-L/beta-2 involved in memory T-cell and neutrophil transmigration.

1. Ebnet, K. et al. (2001) The EMBO journal 20, 3738-48. PMID: 11447115
2. Martìn-Padura, I. et al. (1998) The Journal of cell biology 142, 117-27. PMID: 9660867
Примечание For research use only.
Изображения
  • Anti-Mouse CD321/F11R Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-F11R polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Mouse lung lysate
    Lane 2: Rat lung lysate

Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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