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Anti-Mouse CD322/JAM2 Polyclonal Antibody (MX993014)

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Aperçu
Numéro de catalogueMX993014
Description
Anti-Mouse CD322/JAM2 Polyclonal Antibody (MX993014) is a rabbit polyclonal antibody detecting CD322 in ELISA, IHC, WB. Suitable for Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesMouse
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Mouse CD322/JAM2 (Arg36-Asn236).
Cible JAM-B, VEJAM, Junctional adhesion molecule B, JAM2, VE-JAM, JAM-2, C21orf43, Vascular endothelial junction-associated molecule, Junctional adhesion molecule 2, CD322
Purification Purified by antigen affinity column.
Numéro d'accès Q9JI59
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Junctional adhesion molecule B (CD322/JAM2) is a ~33 kDa protein. Junctional adhesion protein that mediates heterotypic cell-cell interactions with its cognate receptor JAM3 to regulate different cellular processes. Plays a role in homing and mobilization of hematopoietic stem and progenitor cells within the bone marrow. At the surface of bone marrow stromal cells, it contributes to the retention of the hematopoietic stem and progenitor cells expressing JAM3. Plays a central role in leukocytes extravasation by facilitating not only transmigration but also tethering and rolling of leukocytes along the endothelium. Tethering and rolling of leukocytes are dependent on the binding by JAM2 of the integrin alpha-4/beta-1.

1. Lamagna, C. et al. (2005) Molecular biology of the cell 16, 4992-5003. PMID: 16093349
2. Arcangeli, ML. et al. (2011) Blood 118, 4609-19. PMID: 21868569
3. Arcangeli, ML. et al. (2014) Stem cells (Dayton, Ohio) 32, 1043-54. PMID: 24357068
4. Ludwig, RJ. et al. (2005) The Journal of investigative dermatology 125, 969-76. PMID: 16297198
5. Ludwig, RJ. et al. (2009) Immunology 128, 196-205. PMID: 19740376
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Mouse CD322/JAM2 Polyclonal Antibody

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