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Anti-Mouse CD36 Polyclonal Antibody (MB030014)

Anti-Mouse CD36 Polyclonal Antibody
Anti-Mouse CD36 Polyclonal Antibody
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Vista general
Número de catálogoMB030014
Reactividad de especiesMouse, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Mouse CD36 (Lys56-Gly423).
Objetivo Cd36
Purificación Purified by antigen affinity column.
Número de acceso Q08857
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Platelet glycoprotein 4 (CD36) is a ~52 kDa protein. Multifunctional glycoprotein that acts as a receptor for a broad range of ligands. Ligands can be of proteinaceous nature like thrombospondin, fibronectin, collagen or amyloid-beta as well as of lipidic nature such as oxidized low-density lipoprotein (oxLDL), anionic phospholipids, long-chain fatty acids and bacterial diacylated lipopeptides. They are generally multivalent and can therefore engage multiple receptors simultaneously, the resulting formation of CD36 clusters initiates signal transduction and internalization of receptor-ligand complexes. The dependency on coreceptor signaling is strongly ligand specific. Cellular responses to these ligands are involved in angiogenesis, inflammatory response, fatty acid metabolism, taste and dietary fat processing in the intestine (Probable).

1. Endemann, G. et al. (1993) The Journal of biological chemistry 268, 11811-6. PMID: 7685021
2. Jimenez-Dalmaroni, MJ. et al. (2009) PloS one 4, e7411. PMID: 19847289
3. Stewart, CR. et al. (2010) Nature immunology 11, 155-61. PMID: 20037584
4. Heit, B. et al. (2013) Developmental cell 24, 372-83. PMID: 23395392
5. Wang, J. et al. (2019) Cell reports 26, 209-221.e5. PMID: 30605677
6. Hao, JW. et al. (2020) Nature communications 11, 4765. PMID: 32958780
7. Nassir, F. et al. (2007) The Journal of biological chemistry 282, 19493-501. PMID: 17507371
8. Masuda, D. et al. (2009) Journal of lipid research 50, 999-1011. PMID: 18753675
9. Tran, TT. et al. (2011) The Journal of biological chemistry 286, 25201-10. PMID: 21610069
10. Laugerette, F. et al. (2005) The Journal of clinical investigation 115, 3177-84. PMID: 16276419
Nota For research use only.
Imágenes
  • Anti-Mouse CD36 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-CD36 polyclonal antibody at 1µg/ml on various samples.
    Lane 1: Mouse lung lysate
    Lane 2: Mouse heart lysate

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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