Anti-Mouse CD3 epsilon Recombinant Antibody (145-2C11) (MY057207)

Name: Anti-Mouse CD3 epsilon Recombinant Antibody (145-2C11)
Brand: abinScience
SKU: MY057207
Price: 196 USD
Availability: InStock

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号	MY057207
種反応性	Mouse
アプリケーション	FCM
宿主種	Rabbit
クローン性	Monoclonal
アイソタイプ	IgG
クローンID	145-2C11
ターゲット	CD3E, CD3e, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E
エンドトキシンレベル	< 10 EU/mg
純度	>95% as determined by SDS-PAGE.
精製	Protein A/G purified from cell culture supernatant.
アクセッション番号	P22646
形態	Liquid
保存バッファー	0.01M PBS pH 7.4. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
安定性と保存	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景	T-cell surface glycoprotein CD3 epsilon chain (CD3E) is a ~21 kDa protein. Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. CD3E ITAM phosphorylation creates docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme. CD3E is the therapeutic target of blinatumomab (Blincyto). 1. DeJarnette, JB. et al. (1998) Proceedings of the National Academy of Sciences of the United States of America 95, 14909-14. PMID: 9843989 2. Saint-Ruf, C. et al. (2000) Nature 406, 524-7. PMID: 10952314 3. Wang, Y. et al. (2009) PLoS biology 7, e1000253. PMID: 19956738 4. Borroto, A. et al. (2014) Journal of immunology (Baltimore, Md. : 1950) 192, 2042-53. PMID: 24470497 5. Bettini, ML. et al. (2014) Journal of immunology (Baltimore, Md. : 1950) 193, 258-67. PMID: 24899501 6. Du, J. et al. (2025) Cell 188, 4025-4042.e20. PMID: 40592325
注意事項	For research use only.

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Bioactivity

SEC-HPLC detection for Anti-Mouse CD3E Antibody (145-2C11).
Immunofluorescence

Immunofluorescence staining of mouse spleen cells with Rabbit monoclonal antibody (20ug/ml, overnight at 4°C), followed by Alexa Fluor-488-conjugated secondary antibody (6.5ug/ml, 50minutes at room temperature). The target protein was detected in the cell membrane.