中文
English
한국어
日本語
Español
Français
Русский
| カタログ番号 | MY057407 |
|---|---|
| 種反応性 | Mouse |
| アプリケーション | FCM, ICC, IHC, IP, T cell stimulation (Reported), T cell depletion (Reported), WB |
| 宿主種 | Hamster |
| クローン性 | Monoclonal |
| アイソタイプ | IgG |
| クローンID | 145-2C11 |
| ターゲット | CD3E, CD3e, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E |
| エンドトキシンレベル | < 10 EU/mg |
| 純度 | >95% as determined by SDS-PAGE. |
| 精製 | Protein A/G purified from cell culture supernatant. |
| アクセッション番号 | P22646 |
| 形態 | Liquid |
| 保存バッファー | 0.01M PBS pH 7.4. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 背景 | T-cell surface glycoprotein CD3 epsilon chain (CD3E) is a ~21 kDa protein. Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. CD3E ITAM phosphorylation creates docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme. CD3E is the therapeutic target of blinatumomab (Blincyto). 1. DeJarnette, JB. et al. (1998) Proceedings of the National Academy of Sciences of the United States of America 95, 14909-14. PMID: 9843989 2. Saint-Ruf, C. et al. (2000) Nature 406, 524-7. PMID: 10952314 3. Wang, Y. et al. (2009) PLoS biology 7, e1000253. PMID: 19956738 4. Borroto, A. et al. (2014) Journal of immunology (Baltimore, Md. : 1950) 192, 2042-53. PMID: 24470497 5. Bettini, ML. et al. (2014) Journal of immunology (Baltimore, Md. : 1950) 193, 258-67. PMID: 24899501 6. Du, J. et al. (2025) Cell 188, 4025-4042.e20. PMID: 40592325 |
| 注意事項 | For research use only. |
SEC-HPLC detection for Anti-Mouse CD3E Antibody (145-2C11).
SDS-PAGE for Anti-Mouse CD3E Antibody (145-2C11)
+86-027-65523339
中国武漢市深敦寺路666号C棟、武漢、430206
