Anti-Mouse CD3 epsilon Recombinant Antibody (500A2), PE (MY057327)

Name: Anti-Mouse CD3 epsilon Recombinant Antibody (500A2), PE
Brand: abinScience
SKU: MY057327
Price: 214 USD
Availability: InStock

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Numéro de catalogue	MY057327
Reactivité des espèces	Mouse
Applications	FCM
Espèce hôte	Hamster
Clonalité	Monoclonal
Isotype	IgG1
ID de clone	500A2
Conjugaison	PE
Cible	CD3E, CD3e, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Numéro d'accès	P22646
Forme	Liquid
Tampon de stockage	0.01M PBS, pH 7.4, 50% glycerol, 0.05% Proclin 300. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Stabilité et stockage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Contexte	T-cell surface glycoprotein CD3 epsilon chain (CD3E) is a ~21 kDa protein. Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. CD3E ITAM phosphorylation creates docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme. CD3E is the therapeutic target of blinatumomab (Blincyto). 1. DeJarnette, JB. et al. (1998) Proceedings of the National Academy of Sciences of the United States of America 95, 14909-14. PMID: 9843989 2. Saint-Ruf, C. et al. (2000) Nature 406, 524-7. PMID: 10952314 3. Wang, Y. et al. (2009) PLoS biology 7, e1000253. PMID: 19956738 4. Borroto, A. et al. (2014) Journal of immunology (Baltimore, Md. : 1950) 192, 2042-53. PMID: 24470497 5. Bettini, ML. et al. (2014) Journal of immunology (Baltimore, Md. : 1950) 193, 258-67. PMID: 24899501 6. Du, J. et al. (2025) Cell 188, 4025-4042.e20. PMID: 40592325
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.