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Anti-Mouse CD40/TNFRSF5 Recombinant Antibody (FGK45), FITC (MB782117)

Anti-Mouse CD40/TNFRSF5 Recombinant Antibody (FGK45), FITC
Anti-Mouse CD40/TNFRSF5 Recombinant Antibody (FGK45), FITC
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  • 100T
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Vista general
Número de catálogoMB782117
Reactividad de especiesMouse
AplicacionesFCM
Especie huéspedRat
ClonalidadMonoclonal
IsotipoIgG2a, kappa
ID de clonFGK45
ConjugaciónFITC
Aplicaciones probadas FCM
Objetivo B-cell surface antigen CD40, Bp50, CD40, CD40L receptor, CDw40, TNFRSF5, Tumor necrosis factor receptor superfamily member 5
Nivel de endotoxinas Please contact with the lab for this information.
Pureza >90% as determined by SDS-PAGE.
Productos asociados Rat IgG2a, kappa Recombinant Isotype Control Antibody (RTK2758), FITC
Número de acceso P27512
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Estabilidad y almacenamiento Store at 4°C for 12 months. Protect from light. Do not freeze.
Fondo

Tumor necrosis factor receptor superfamily member 5 (CD40/TNFRSF5) is a ~32 kDa protein. Receptor for TNFSF5/CD40LG. Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion.

1. Eliopoulos, AG. et al. (2003) The EMBO journal 22, 3855-64. PMID: 12881420
Nota For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
Imágenes
  • Anti-Mouse CD40/TNFRSF5 Recombinant Antibody (FGK45), FITC

    Flow CytoMetry

    Flow-cytometry using FITC anti-Mouse CD40 antibody. Mouse spleen cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-Mouse CD40 monoclonal antibody (Catalog: MB782117, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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