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Anti-Mouse CD45 Recombinant Antibody (SAA0570) (MY195107)

Anti-Mouse CD45 Recombinant Antibody (SAA0570)
Anti-Mouse CD45 Recombinant Antibody (SAA0570)
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Aperçu
Numéro de catalogueMY195107
Reactivité des espècesMouse
ApplicationsFCM
Espèce hôteMouse
ClonalitéMonoclonal
IsotypeVHH, mFc (IgG2a)
ID de cloneSAA0570
Applications testées FCM
Cible T200, Leukocyte common antigen, CD45, L-CA, Receptor-type tyrosine-protein phosphatase C, PTPRC
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Numéro d'accès P06800
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 0.09% Sodium azide.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Contexte

Receptor-type tyrosine-protein phosphatase C (CD45/PTPRC) is a ~144 kDa protein. Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity.

Note For research use only.
Images
  • Anti-Mouse CD45 Recombinant Antibody (SAA0570)

    Flow Cytometry

    Flow-cytometry using anti-mouse CD45 antibody.Raw264.7 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-mouse CD45 antibody monoclonal antibody (Catalog # MY195107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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