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Anti-Mouse CD45 Recombinant Antibody (SAA0570) (MY195107)

Anti-Mouse CD45 Recombinant Antibody (SAA0570)
Anti-Mouse CD45 Recombinant Antibody (SAA0570)
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  • データシート

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概要
カタログ番号MY195107
種反応性Mouse
アプリケーションFCM
宿主種Mouse
クローン性Monoclonal
アイソタイプVHH, mFc (IgG2a)
クローンIDSAA0570
テスト済みアプリケーション FCM
ターゲット T200, Leukocyte common antigen, CD45, L-CA, Receptor-type tyrosine-protein phosphatase C, PTPRC
エンドトキシンレベル Please contact with the lab for this information.
純度 >95% as determined by SDS-PAGE.
精製 Protein A/G purified from cell culture supernatant.
アクセッション番号 P06800
形態 Liquid
保存バッファー 0.01M PBS, pH 7.4, 0.09% Sodium azide.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

安定性と保存 Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景

Receptor-type tyrosine-protein phosphatase C (CD45/PTPRC) is a ~144 kDa protein. Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity.

注意事項 For research use only.
イメージ
  • Anti-Mouse CD45 Recombinant Antibody (SAA0570)

    Flow Cytometry

    Flow-cytometry using anti-mouse CD45 antibody.Raw264.7 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-mouse CD45 antibody monoclonal antibody (Catalog # MY195107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

参考文献
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