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Anti-Mouse CD45 Recombinant Antibody (SAA0570)
Anti-Mouse CD45 Recombinant Antibody (SAA0570)
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Спецификация:
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  • 100ug
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  • Обзор

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  • Даташит

  • SDS

Обзор
Каталожный номерMY195107
Реактивность видовMouse
ПримененияFCM
Хозяинский видMouse
КлоональностьMonoclonal
ИзотипVHH, mFc (IgG2a)
ID клонаSAA0570
Проверенные приложения FCM
Цель T200, Leukocyte common antigen, CD45, L-CA, Receptor-type tyrosine-protein phosphatase C, PTPRC
Уровень эндотоксинов Please contact with the lab for this information.
Чистота >95% as determined by SDS-PAGE.
Очистка Protein A/G purified from cell culture supernatant.
Номер доступа P06800
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 0.09% Sodium azide.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Фон

Receptor-type tyrosine-protein phosphatase C (CD45/PTPRC) is a ~144 kDa protein. Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity.

Примечание For research use only.
Изображения
  • Anti-Mouse CD45 Recombinant Antibody (SAA0570)

    Flow Cytometry

    Flow-cytometry using anti-mouse CD45 antibody.Raw264.7 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-mouse CD45 antibody monoclonal antibody (Catalog # MY195107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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