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Anti-Mouse CD45 Polyclonal Antibody (MY195014)

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Anti-Mouse CD45 Polyclonal Antibody
Anti-Mouse CD45 Polyclonal Antibody
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Aperçu
Numéro de catalogueMY195014
Description
Anti-Mouse CD45/PTPRC Polyclonal Antibody (MY195014) is a rabbit polyclonal antibody detecting Ptprc in ELISA, IHC, WB. Suitable for Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesMouse
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Mouse CD45/PTPRC (Thr202-Phe563).
Applications testées WB
Cible Receptor-type tyrosine-protein phosphatase C, 3.1.3.48, Leukocyte common antigen, L-CA, Lymphocyte antigen 5, Ly-5, T200, CD45, Ptprc, Ly-5
Purification Purified by antigen affinity column.
Numéro d'accès P06800
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Receptor-type tyrosine-protein phosphatase C (CD45/PTPRC) is a ~144 kDa protein. Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity.

Note For research use only.
Images
  • Anti-Mouse CD45 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-PTPRC polyclonal antibody at 1ug/mL on various samples.
    Lane 1: RAW264.7 cell lysate
    Lane 2: Mouse spleen lysate
    Lane 3: Mouse thymus lysate

Reférences
Fiche technique
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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