Anti-Mouse CD69 Polyclonal Antibody (MC273024)

Name: Anti-Mouse CD69 Polyclonal Antibody
Brand: abinScience
SKU: MC273024
Price: 96 USD
Availability: InStock

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SDS

Aperçu

Numéro de catalogue

MC273024

Description

Anti-Mouse CD69 Polyclonal Antibody (MC273024) is a rabbit polyclonal antibody detecting CD69 in ELISA, IHC, WB. Suitable for Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

Mouse

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Mouse CD69 (Asn62-Arg199).

Applications testées

Cible

Early activation antigen CD69, CD69, Cd69

Niveau d'endotoxines

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Numéro d'accès

P37217

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Early activation antigen CD69 is a ~22 kDa protein. Transmembrane protein expressed mainly on T-cells resident in mucosa that plays an essential role in immune cell homeostasis. Rapidly expressed on the surface of platelets, T-lymphocytes and NK cells upon activation by various stimuli, such as antigen recognition or cytokine signaling, stimulates different signaling pathways in different cell types. Negatively regulates Th17 cell differentiation through its carbohydrate dependent interaction with galectin-1/LGALS1 present on immature dendritic cells. Association of CD69 cytoplasmic tail with the JAK3/STAT5 signaling pathway regulates the transcription of RORgamma/RORC and, consequently, differentiation toward the Th17 lineage. Also acts via the S100A8/S100A9 complex present on peripheral blood mononuclear cells to promote the conversion of naive CD4 T-cells into regulatory T-cells.

1. Martín, P. et al. (2010) Molecular and cellular biology 30, 4877-89. PMID: 20696842

2. Bui, DV. et al. (2024) Biomolecules 14. PMID: 38540778

3. Shiow, LR. et al. (2006) Nature 440, 540-4. PMID: 16525420

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-CD69 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse spleen lysate
Lane 2: Mouse lung lysate
Lane 3: Rat thymus lysate