Anti-Mouse CD69 Polyclonal Antibody (MC273024)

Name: Anti-Mouse CD69 Polyclonal Antibody
Brand: abinScience
SKU: MC273024
Price: 96 USD
Availability: InStock

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Обзор

Каталожный номер

MC273024

Описание

Anti-Mouse CD69 Polyclonal Antibody (MC273024) is a rabbit polyclonal antibody detecting CD69 in ELISA, IHC, WB. Suitable for Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Реактивность видов

Mouse

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Mouse CD69 (Asn62-Arg199).

Проверенные приложения

Цель

Early activation antigen CD69, CD69, Cd69

Уровень эндотоксинов

Please contact with the lab for this information.

Очистка

Purified by antigen affinity column.

Номер доступа

P37217

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Early activation antigen CD69 is a ~22 kDa protein. Transmembrane protein expressed mainly on T-cells resident in mucosa that plays an essential role in immune cell homeostasis. Rapidly expressed on the surface of platelets, T-lymphocytes and NK cells upon activation by various stimuli, such as antigen recognition or cytokine signaling, stimulates different signaling pathways in different cell types. Negatively regulates Th17 cell differentiation through its carbohydrate dependent interaction with galectin-1/LGALS1 present on immature dendritic cells. Association of CD69 cytoplasmic tail with the JAK3/STAT5 signaling pathway regulates the transcription of RORgamma/RORC and, consequently, differentiation toward the Th17 lineage. Also acts via the S100A8/S100A9 complex present on peripheral blood mononuclear cells to promote the conversion of naive CD4 T-cells into regulatory T-cells.

1. Martín, P. et al. (2010) Molecular and cellular biology 30, 4877-89. PMID: 20696842

2. Bui, DV. et al. (2024) Biomolecules 14. PMID: 38540778

3. Shiow, LR. et al. (2006) Nature 440, 540-4. PMID: 16525420

Примечание

For research use only

Изображения

Western blot

Western blot analysis was performed using anti-CD69 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse spleen lysate
Lane 2: Mouse lung lysate
Lane 3: Rat thymus lysate