Anti-Mouse CD8 alpha Recombinant Antibody (2.43), FITC (MF815117)

Name: Anti-Mouse CD8 alpha Recombinant Antibody (2.43), FITC
Brand: abinScience
SKU: MF815117
Price: 196 USD
Availability: InStock

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Número de catálogo	MF815117
Reactividad de especies	Mouse
Aplicaciones	FCM
Especie huésped	Rat
Clonalidad	Monoclonal
Isotipo	lgG2b
ID de clon	2.43
Conjugación	FITC
Aplicaciones probadas	FCM
Objetivo	CD8a, Cd8a, Lyt-2, Lyt2, T-cell surface glycoprotein CD8 alpha chain, T-cell surface glycoprotein Lyt-2
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Número de acceso	P01731
Forma	Liquid
Buffer de almacenamiento	0.01M PBS, pH 7.4, 50% glycerol, 0.05% Proclin 300. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Estabilidad y almacenamiento	Store at 4°C for 12 months. Protect from light. Do not freeze.
Fondo	T-cell surface glycoprotein CD8 alpha chain (CD8a/Lyt2) is a ~27 kDa protein. Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex.
Nota	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

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Flow CytoMetry

Flow-cytometry using FITC anti-Mouse CD8a antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-Mouse CD8a monoclonal antibody (Catalog: MF815117, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

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Datasheet