Anti-Mouse CD8 alpha Recombinant Antibody (2.43), FITC (MF815117)

Name: Anti-Mouse CD8 alpha Recombinant Antibody (2.43), FITC
Brand: abinScience
SKU: MF815117
Price: 196 USD
Availability: InStock

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概要
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参考文献
データシート
SDS

概要

カタログ番号	MF815117
種反応性	Mouse
アプリケーション	FCM
宿主種	Rat
クローン性	Monoclonal
アイソタイプ	lgG2b
クローンID	2.43
抱合	FITC
テスト済みアプリケーション	FCM
ターゲット	CD8a, Cd8a, Lyt-2, Lyt2, T-cell surface glycoprotein CD8 alpha chain, T-cell surface glycoprotein Lyt-2
エンドトキシンレベル	Please contact with the lab for this information.
純度	>90% as determined by SDS-PAGE.
アクセッション番号	P01731
形態	Liquid
保存バッファー	0.01M PBS, pH 7.4, 50% glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
安定性と保存	Store at 4°C for 12 months. Protect from light. Do not freeze.
背景	T-cell surface glycoprotein CD8 alpha chain (CD8a/Lyt2) is a ~27 kDa protein. Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex.
注意事項	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

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Flow CytoMetry

Flow-cytometry using FITC anti-Mouse CD8a antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-Mouse CD8a monoclonal antibody (Catalog: MF815117, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

参考文献

データシート