Anti-Mouse CD8B//Lyt3 Recombinant Antibody (YTS156.7.7), APC (MY433137)

Name: Anti-Mouse CD8B//Lyt3 Recombinant Antibody (YTS156.7.7), APC
Brand: abinScience
SKU: MY433137
Price: 256 USD
Availability: InStock

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Aperçu

Numéro de catalogue	MY433137
Reactivité des espèces	Mouse
Applications	FCM
Espèce hôte	Rat
Clonalité	Monoclonal
Isotype	IgG2b, kappa
ID de clone	YTS156.7.7
Conjugaison	APC
Cible	T-cell surface glycoprotein CD8 beta chain, Lymphocyte antigen 3, T-cell membrane glycoprotein Ly-3, T-cell surface glycoprotein Lyt-3, CD8b, Cd8b1, Ly-3, Lyt-3, Lyt3, cd8
Niveau d'endotoxines	Please contact with the lab for this information.
Produits associés	Rat IgG2b, kappa Recombinant Isotype Control Antibody (ES26-5E12.4), APC
Numéro d'accès	P10300
Forme	Liquid
Tampon de stockage	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Stabilité et stockage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Contexte	T-cell surface glycoprotein CD8 beta chain (CD8b/Lyt3) is a ~24 kDa protein. Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex.
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

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Flow Cytometry

Flow-cytometry using APC anti-Mouse CD8b antibody. Mouse spleen cells were stained with an irrelevant antibody (Green Histogram) or an APC anti-Mouse CD8b antibody monoclonal antibody (Catalog # MY433137 , Red Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

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