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Anti-Mouse CD9 Polyclonal Antibody (MB984014)

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Aperçu
Numéro de catalogueMB984014
Description
Anti-Mouse CD9 Polyclonal Antibody (MB984014) is a rabbit polyclonal antibody detecting CD9 in ELISA, IHC, WB. Suitable for Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesMouse
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Mouse CD9 (Thr110-Ile193).
Cible CD9 antigen, CD9, Cd9
Purification Purified by antigen affinity column.
Numéro d'accès P40240
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

CD9 antigen is a ~25 kDa protein. Integral membrane protein associated with integrins, which regulates different processes, such as sperm-egg fusion, platelet activation and aggregation, and cell adhesion. Present at the cell surface of oocytes and plays a key role in sperm-egg fusion, possibly by organizing multiprotein complexes and the morphology of the membrane required for the fusion. In myoblasts, associates with CD81 and PTGFRN and inhibits myotube fusion during muscle regeneration. In macrophages, associates with CD9 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles. Also prevents the fusion between mononuclear cell progenitors into osteoclasts in charge of bone resorption.

1. Le Naour, F. et al. (2000) Science (New York, N.Y.) 287, 319-21. PMID: 10634790
2. Miyado, K. et al. (2000) Science (New York, N.Y.) 287, 321-4. PMID: 10634791
3. Kaji, K. et al. (2000) Nature genetics 24, 279-82. PMID: 10700183
4. Ishibashi, T. et al. (2004) The Journal of neuroscience : the official journal of the Society for Neuroscience 24, 96-102. PMID: 14715942
5. Jégou, A. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 10946-51. PMID: 21690351
6. Charrin, S. et al. (2013) Nature communications 4, 1674. PMID: 23575678
7. Takeda, Y. et al. (2003) The Journal of cell biology 161, 945-56. PMID: 12796480
9. Takeda, Y. et al. (2008) The Journal of biological chemistry 283, 26089-97. PMID: 18662991
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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