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Обзор
Каталожный номерMB984014
Описание
Anti-Mouse CD9 Polyclonal Antibody (MB984014) is a rabbit polyclonal antibody detecting CD9 in ELISA, IHC, WB. Suitable for Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Реактивность видовMouse
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Mouse CD9 (Thr110-Ile193).
Цель CD9 antigen, CD9, Cd9
Очистка Purified by antigen affinity column.
Номер доступа P40240
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

CD9 antigen is a ~25 kDa protein. Integral membrane protein associated with integrins, which regulates different processes, such as sperm-egg fusion, platelet activation and aggregation, and cell adhesion. Present at the cell surface of oocytes and plays a key role in sperm-egg fusion, possibly by organizing multiprotein complexes and the morphology of the membrane required for the fusion. In myoblasts, associates with CD81 and PTGFRN and inhibits myotube fusion during muscle regeneration. In macrophages, associates with CD9 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles. Also prevents the fusion between mononuclear cell progenitors into osteoclasts in charge of bone resorption.

1. Le Naour, F. et al. (2000) Science (New York, N.Y.) 287, 319-21. PMID: 10634790
2. Miyado, K. et al. (2000) Science (New York, N.Y.) 287, 321-4. PMID: 10634791
3. Kaji, K. et al. (2000) Nature genetics 24, 279-82. PMID: 10700183
4. Ishibashi, T. et al. (2004) The Journal of neuroscience : the official journal of the Society for Neuroscience 24, 96-102. PMID: 14715942
5. Jégou, A. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 10946-51. PMID: 21690351
6. Charrin, S. et al. (2013) Nature communications 4, 1674. PMID: 23575678
7. Takeda, Y. et al. (2003) The Journal of cell biology 161, 945-56. PMID: 12796480
9. Takeda, Y. et al. (2008) The Journal of biological chemistry 283, 26089-97. PMID: 18662991
Примечание For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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