Anti-Mouse IL-10 R alpha Recombinant Antibody (1B1.3A) (MC614013)

Name: Anti-Mouse IL-10 R alpha Recombinant Antibody (1B1.3A)
Brand: abinScience
SKU: MC614013
Price: 191 USD
Availability: InStock

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Spécification :

50ug
100ug
1mg

Nombre :

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

MC614013

Reactivité des espèces

Mouse

Applications

ELISA, FCM, Functional assay, Research in vivo

Espèce hôte

Rat

Clonalité

Monoclonal

Isotype

IgG1, kappa

ID de clone

1B1.3A

Cible

CD210, CDw210a, IL-10 receptor subunit alpha, IL-10R subunit 1, IL-10R subunit alpha, IL-10R1, IL-10RA, IL10R, IL10RA, Interleukin-10 receptor subunit 1, Interleukin-10 receptor subunit alpha

Niveau d'endotoxines

< 10 EU/mg

Pureté

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Numéro d'accès

Q61727

Forme

Liquid

Tampon de stockage

0.01M PBS pH 7.4.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:2000-1:20000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Interleukin-10 receptor subunit alpha (CDw210a/IL10RA) is a ~64 kDa protein. Cell surface receptor for the cytokine IL10 that participates in IL10-mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Upon binding to IL10, induces a conformational change in IL10RB, allowing IL10RB to bind IL10 as well. In turn, the heterotetrameric assembly complex, composed of two subunits of IL10RA and IL10RB, activates the kinases JAK1 and TYK2 that are constitutively associated with IL10RA and IL10RB respectively. These kinases then phosphorylate specific tyrosine residues in the intracellular domain in IL10RA leading to the recruitment and subsequent phosphorylation of STAT3. Once phosphorylated, STAT3 homodimerizes, translocates to the nucleus and activates the expression of anti-inflammatory genes.

1. Weber-Nordt, RM. et al. (1996) The Journal of biological chemistry 271, 27954-61. PMID: 8910398

Note

For research use only

Images

SDS-PAGE

SDS-PAGE for Anti-Mouse CDw210a/IL10RA Recombinant Antibody (1B1.3A)

Reférences

Fiche technique

Anti-Mouse IL-10 R alpha Recombinant Antibody (1B1.3A).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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