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Anti-Mouse Cathepsin B Polyclonal Antibody (MY436014)

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Anti-Mouse Cathepsin B Polyclonal Antibody
Anti-Mouse Cathepsin B Polyclonal Antibody
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Aperçu
Numéro de catalogueMY436014
Description
Anti-Mouse Cathepsin B Polyclonal Antibody (MY436014) is a rabbit polyclonal antibody detecting Cathepsin B in ELISA, IHC, WB. Suitable for Mouse, Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesMouse, Rat
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Mouse CTSB (Asp51-Phe339).
Cible Cathepsin B (EC 3.4.22.1) (Cathepsin B1) [Cleaved into: Cathepsin B light chain, Cathepsin B heavy chain]
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >95% as determined by SDS-PAGE.
Purification Purified by antigen affinity column.
Numéro d'accès P10605
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Cathepsin B (CTSB) is a ~37 kDa protein. Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Cleaves matrix extracellular phosphoglycoprotein MEPE. Involved in the solubilization of cross-linked TG/thyroglobulin in the thyroid follicle lumen. Has also been implicated in tumor invasion and metastasis.

1. Friedrichs, B. et al. (2003) The Journal of clinical investigation 111, 1733-45. PMID: 12782676
Note For research use only.
Images
  • Anti-Mouse Cathepsin B Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-CTSB polyclonal antibody at 1µg/ml on various samples.
    Lane 1: RAW264.7 cell lysate

Reférences
Fiche technique
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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