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Anti-Mouse EGFLAM Polyclonal Antibody (MP294014)

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Vista general
Número de catálogoMP294014
Descripción
Anti-Mouse EGFLAM Polyclonal Antibody (MP294014) is a rabbit polyclonal antibody detecting EGFLAM in ELISA, IHC, WB. Suitable for Mouse, Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesMouse, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Mouse EGFLAM (Asp40-Thr236).
Objetivo Pikachurin (EGF-like, fibronectin type-III and laminin G-like domain-containing protein) (Nectican)
Purificación Purified by antigen affinity column.
Número de acceso Q4VBE4
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Pikachurin (EGFLAM) is a ~110 kDa protein. Involved in both the retinal photoreceptor ribbon synapse formation and physiological functions of visual perception. Plays a key role in the synaptic organization of photoreceptors by mediating transsynaptic interaction between alpha-dystroglycan and GPR179 on the postsynaptic membrane. Necessary for proper bipolar dendritic tip apposition to the photoreceptor ribbon synapse. Promotes matrix assembly and cell adhesiveness.

1. Sato, S. et al. (2008) Nature neuroscience 11, 923-31. PMID: 18641643
2. Manabe, R. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 12849-54. PMID: 18757743
3. Orlandi, C. et al. (2018) Cell reports 25, 130-145.e5. PMID: 30282023
Nota For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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