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| カタログ番号 | MF004137 |
|---|---|
| 種反応性 | Mouse |
| アプリケーション | ELISA, FCM, WB |
| 宿主種 | Rabbit |
| クローン性 | Monoclonal |
| アイソタイプ | IgG |
| クローンID | SAA2220 |
| 抱合 | PE |
| ターゲット | EC:2.7.10.1, EGFR, ERBB, ERBB1, Epidermal growth factor receptor, HER1, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1 |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >95% as determined by SDS-PAGE. |
| 関連製品 | Rabbit IgG Recombinant Isotype Control Antibody (HyHEL-10), PE |
| 精製 | Affinity chromatography. |
| アクセッション番号 | Q01279 |
| 形態 | Liquid |
| 保存バッファー | 0.01M PBS, pH 7.4, 50% glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 安定性と保存 | Store at 4°C for 12 months. Protect from light. Do not freeze. |
| 背景 | Epidermal growth factor receptor (EGFR/ERBB1) is a ~134 kDa protein. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. EGFR is the therapeutic target of cetuximab (Erbitux) and panitumumab (Vectibix). 1. Fazioli, F. et al. (1993) The EMBO journal 12, 3799-808. PMID: 8404850 2. Peng, SC. et al. (2010) Nucleic acids research 38, 7446-57. PMID: 20639532 3. Eagles, DA. et al. (2022) Proceedings of the National Academy of Sciences of the United States of America 119. PMID: 35131940 |
| 注意事項 | For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only. |
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