Anti-Mouse GDF-15 Polyclonal Antibody (MV986014)

Name: Anti-Mouse GDF-15 Polyclonal Antibody
Brand: abinScience
SKU: MV986014
Price: 96 USD
Availability: InStock

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SDS

Aperçu

Numéro de catalogue

MV986014

Description

Anti-Mouse GDF-15 Polyclonal Antibody (MV986014) is a rabbit polyclonal antibody detecting GDF-15 in ELISA, IHC, WB. Suitable for Mouse, Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Mouse GDF15/MIC1 (Gly203-Ala303).

Cible

Growth/differentiation factor 15, GDF-15, Macrophage inhibitory cytokine 1, MIC-1

Niveau d'endotoxines

Please contact with the lab for this information.

Pureté

>95% as determined by SDS-PAGE.

Purification

Purified by antigen affinity column.

Numéro d'accès

Q9Z0J7

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Growth/differentiation factor 15 (GDF15/MIC1) is a ~33 kDa protein. Hormone produced in response to various stresses to confer information about those stresses to the brain, and trigger an aversive response, characterized by nausea and/or loss of appetite. The aversive response is both required to reduce continuing exposure to those stresses at the time of exposure and to promote avoidance behavior in the future. Acts by binding to its receptor, GFRAL, activating GFRAL-expressing neurons localized in the area postrema and nucleus tractus solitarius of the brainstem. It then triggers the activation of neurons localized within the parabrachial nucleus and central amygdala, which constitutes part of the 'emergency circuit' that shapes responses to stressful conditions. The GDF15-GFRAL signal induces expression of genes involved in metabolism, such as lipid metabolism in adipose tissues.

1. Tsai, VW. et al. (2013) PloS one 8, e55174. PMID: 23468844

2. Chung, HK. et al. (2017) The Journal of cell biology 216, 149-165. PMID: 27986797

3. Wang, T. et al. (2017) EMBO molecular medicine 9, 1150-1164. PMID: 28572090

4. Mullican, SE. et al. (2017) Nature medicine 23, 1150-1157. PMID: 28846097

5. Emmerson, PJ. et al. (2017) Nature medicine 23, 1215-1219. PMID: 28846098

6. Yang, L. et al. (2017) Nature medicine 23, 1158-1166. PMID: 28846099

7. Hsu, JY. et al. (2017) Nature 550, 255-259. PMID: 28953886

8. Tsai, VW. et al. (2018) International journal of obesity (2005) 42, 561-571. PMID: 29026214

9. Xiong, Y. et al. (2017) Science translational medicine 9. PMID: 29046435

10. Patel, S. et al. (2019) Cell metabolism 29, 707-718.e8. PMID: 30639358

Note

For research use only.

Images

Reférences

Fiche technique

Anti-Mouse GDF-15 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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