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Anti-Mouse GLI2 Polyclonal Antibody (MY480014)

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Vista general
Número de catálogoMY480014
Descripción
Anti-Mouse GLI2 Polyclonal Antibody (MY480014) is a rabbit polyclonal antibody detecting Gli2 in ELISA, IHC, WB. Suitable for Human, Mouse, Drosophila yakuba, Chicken, and Pan troglodytes.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Mouse GLI2 (Glu416-Gly570).
Objetivo Zinc finger protein GLI2, Tax helper protein, Gli2, Thp
Purificación Purified by antigen affinity column.
Número de acceso Q0VGT2
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Zinc finger protein GLI2 is a ~165 kDa protein. Functions as a transcription regulator in the hedgehog (Hh) pathway. Functions as a transcriptional activator. May also function as transcriptional repressor. Requires STK36 for full transcriptional activator activity. Binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element.

1. Mo, R. et al. (1997) Development (Cambridge, England) 124, 113-23. PMID: 9006072
2. Murone, M. et al. (2000) Nature cell biology 2, 310-2. PMID: 10806483
3. Sasaki, H. et al. (1999) Development (Cambridge, England) 126, 3915-24. PMID: 10433919
Nota For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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