Anti-Mouse Glypican 3 Recombinant Antibody (7C8) (MW310013)

Name: Anti-Mouse Glypican 3 Recombinant Antibody (7C8)
Brand: abinScience
SKU: MW310013
Price: 126 USD
Availability: InStock

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Precio(USD):

Especificación:

20ug
50ug
100ug
1mg

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

MW310013

Reactividad de especies

Mouse

Aplicaciones

Blocking, ELISA, FCM

Especie huésped

Rat

Clonalidad

Monoclonal

Isotipo

IgG1

ID de clon

7C8

Aplicaciones probadas

Objetivo

GPC3, MXR7, GTR2-2, Glypican-3, Intestinal protein OCI-5, OCI5

Nivel de endotoxinas

Please contact with the lab for this information.

Pureza

>95% as determined by SDS-PAGE.

Purificación

Protein A/G purified from cell culture supernatant.

Número de acceso

Q8CFZ4

RRID

Anti-Mouse GPC3 Antibody (7C8) (abinScience Cat# MW310013, RRID:AB_3725638)

Forma

Liquid

Buffer de almacenamiento

0.01M PBS pH 7.4

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:2000-1:20000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Fondo

Glypican-3 (GPC3/Glypican 3) is a ~65 kDa protein. Cell surface proteoglycan. Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins. Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation. Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands. Positively regulates the non-canonical Wnt signaling pathway.

1. Capurro, MI. et al. (2008) Developmental cell 14, 700-11. PMID: 18477453

2. Bhave, VS. et al. (2013) The American journal of pathology 183, 153-9. PMID: 23665349

3. Song, HH. et al. (2005) The Journal of biological chemistry 280, 2116-25. PMID: 15537637

4. Paine-Saunders, S. et al. (2000) Developmental biology 225, 179-87. PMID: 10964473

5. Grisaru, S. et al. (2001) Developmental biology 231, 31-46. PMID: 11180950

6. Ng, A. et al. (2009) Developmental biology 335, 208-15. PMID: 19733558

Nota

For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Anti-Mouse GPC3 Antibody (7C8).
Western blot

Lane 1: HepG2 cell lysate

Referencias

Datasheet

Anti-Mouse Glypican 3 Recombinant Antibody (7C8).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo