Anti-Mouse Glypican 3 Recombinant Antibody (7C8) (MW310013)

Name: Anti-Mouse Glypican 3 Recombinant Antibody (7C8)
Brand: abinScience
SKU: MW310013
Price: 126 USD
Availability: InStock

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1mg

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

MW310013

Reactivité des espèces

Mouse

Applications

Blocking, ELISA, FCM

Espèce hôte

Rat

Clonalité

Monoclonal

Isotype

IgG1

ID de clone

7C8

Applications testées

Cible

GPC3, MXR7, GTR2-2, Glypican-3, Intestinal protein OCI-5, OCI5

Niveau d'endotoxines

Please contact with the lab for this information.

Pureté

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Numéro d'accès

Q8CFZ4

RRID

Anti-Mouse GPC3 Antibody (7C8) (abinScience Cat# MW310013, RRID:AB_3725638)

Forme

Liquid

Tampon de stockage

0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:2000-1:20000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Contexte

Glypican-3 (GPC3/Glypican 3) is a ~65 kDa protein. Cell surface proteoglycan. Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins. Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation. Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands. Positively regulates the non-canonical Wnt signaling pathway.

1. Capurro, MI. et al. (2008) Developmental cell 14, 700-11. PMID: 18477453

2. Bhave, VS. et al. (2013) The American journal of pathology 183, 153-9. PMID: 23665349

3. Song, HH. et al. (2005) The Journal of biological chemistry 280, 2116-25. PMID: 15537637

4. Paine-Saunders, S. et al. (2000) Developmental biology 225, 179-87. PMID: 10964473

5. Grisaru, S. et al. (2001) Developmental biology 231, 31-46. PMID: 11180950

6. Ng, A. et al. (2009) Developmental biology 335, 208-15. PMID: 19733558

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Mouse GPC3 Antibody (7C8).
Western blot

Lane 1: HepG2 cell lysate

Reférences

Fiche technique

Anti-Mouse Glypican 3 Recombinant Antibody (7C8).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

Kits d'Essai