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Anti-Mouse GPX1 Polyclonal Antibody
Anti-Mouse GPX1 Polyclonal Antibody
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Обзор
Каталожный номерMY365014
Описание
Anti-Mouse GPX1 Polyclonal Antibody (MY365014) is a rabbit polyclonal antibody detecting Gpx1 in ELISA, IHC, WB. Suitable for Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Реактивность видовMouse, Rat
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Mouse GPX1 (Gly48-Ser201).
Цель Glutathione peroxidase 1, GPx-1, GSHPx-1, 1.11.1.9, Cellular glutathione peroxidase, Phospholipid-hydroperoxide glutathione peroxidase GPX1, 1.11.1.12, Selenium-dependent glutathione peroxidase 1, Gpx1
Очистка Purified by antigen affinity column.
Номер доступа P11352
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Glutathione peroxidase 1 (GPX1) is a ~22 kDa protein. Catalyzes the reduction of hydroperoxides in a glutathione-dependent manner thus regulating cellular redox homeostasis. Can reduce small soluble hydroperoxides such as H2O2, cumene hydroperoxide and tert-butyl hydroperoxide, as well as several fatty acid-derived hydroperoxides. In platelets catalyzes the reduction of 12-hydroperoxyeicosatetraenoic acid, the primary product of the arachidonate 12-lipoxygenase pathway.

1. Esposito, LA. et al. (2000) Free radical biology & medicine 28, 754-66. PMID: 10754271
2. Wang, SK. et al. (2011) Free radical biology & medicine 51, 197-204. PMID: 21420488
3. Schwarz, M. et al. (2023) Redox biology 59, 102593. PMID: 36608588
4. Esworthy, RS. et al. (1997) Archives of biochemistry and biophysics 340, 59-63. PMID: 9126277
5. Ho, YS. et al. (1997) The Journal of biological chemistry 272, 16644-51. PMID: 9195979
6. de Haan, JB. et al. (1998) The Journal of biological chemistry 273, 22528-36. PMID: 9712879
Примечание For research use only.
Изображения
  • Anti-Mouse GPX1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-GPX1 polyclonal antibody at GPX1 on various samples.
    Lane 1: Mouse liver lysate
    Lane 2: Mouse kidney lysate
    Lane 3: Mouse spleen lysate
    Lane 4: Mouse lung lysate
    Lane 5: Rat liver lysate

Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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