Anti-Mouse HMGB1 Recombinant Antibody (DPH1.1) (MY200013)

Name: Anti-Mouse HMGB1 Recombinant Antibody (DPH1.1)
Brand: abinScience
SKU: MY200013
Price: 126 USD
Availability: InStock

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Precio(USD):

Especificación:

20ug
50ug
100ug
1mg

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

MY200013

Marca

abinScience

Reactividad de especies

Bovine, Canine, Equine, Porcine

Aplicaciones

FCM, FuncS, IF, WB

Especie huésped

Rat

Clonalidad

Monoclonal

Isotipo

IgG1, kappa

ID de clon

DPH1.1

Objetivo

High mobility group protein B1, HMGB1, HMG1, HMG-1, High mobility group protein 1

Nivel de endotoxinas

Please contact with the lab for this information.

Pureza

>95% as determined by SDS-PAGE.

Purificación

Protein A/G purified from cell culture supernatant.

Número de acceso

P63158

Forma

Liquid

Buffer de almacenamiento

0.01M PBS pH 7.4

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Reconstitución

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

Información de uso del producto

Aplicación	Dilución
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Fondo

High mobility group protein B1 (HMGB1) is a ~24 kDa protein. Multifunctional redox sensitive protein with various roles in different cellular compartments. In the nucleus is one of the major chromatin-associated non-histone proteins and acts as a DNA chaperone involved in replication, transcription, chromatin remodeling, V(D)J recombination, DNA repair and genome stability. Proposed to be an universal biosensor for nucleic acids. Promotes host inflammatory response to sterile and infectious signals and is involved in the coordination and integration of innate and adaptive immune responses. In the cytoplasm functions as a sensor and/or chaperone for immunogenic nucleic acids implicating the activation of TLR9-mediated immune responses, and mediates autophagy.

1. Yang, H. et al. (2013) Journal of leukocyte biology 93, 865-73. PMID: 23446148

2. Li, G. et al. (2013) Frontiers in immunology 4, 68. PMID: 23519706

3. Li, G. et al. (2013) Seminars in cancer biology 23, 380-90. PMID: 23994764

4. Lee, SA. et al. (2014) Yonsei medical journal 55, 1165-76. PMID: 25048472

5. Mitola, S. et al. (2006) Journal of immunology (Baltimore, Md. : 1950) 176, 12-5. PMID: 16365390

Nota

For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Anti-Mouse HMGB1 Antibody (DPH1.1)
Western blot

Western blot analysis was performed using anti-HMGB1 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human HMGB1 (Catalog No: HY200011)
Lane 2: negative control

Referencias

Datasheet

Anti-Mouse HMGB1 Recombinant Antibody (DPH1.1).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo