Anti-Mouse HMGB1 Recombinant Antibody (DPH1.1) (MY200013)

Name: Anti-Mouse HMGB1 Recombinant Antibody (DPH1.1)
Brand: abinScience
SKU: MY200013
Price: 126 USD
Availability: InStock

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Prix(USD) :

Spécification :

20ug
50ug
100ug
1mg

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

MY200013

Marque

abinScience

Reactivité des espèces

Bovine, Canine, Equine, Porcine

Applications

FCM, FuncS, IF, WB

Espèce hôte

Rat

Clonalité

Monoclonal

Isotype

IgG1, kappa

ID de clone

DPH1.1

Cible

High mobility group protein B1, HMGB1, HMG1, HMG-1, High mobility group protein 1

Niveau d'endotoxines

Please contact with the lab for this information.

Pureté

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Numéro d'accès

P63158

Forme

Liquid

Tampon de stockage

0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Reconstitution

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

Informations d'utilisation du produit

Application	Dilution
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Contexte

High mobility group protein B1 (HMGB1) is a ~24 kDa protein. Multifunctional redox sensitive protein with various roles in different cellular compartments. In the nucleus is one of the major chromatin-associated non-histone proteins and acts as a DNA chaperone involved in replication, transcription, chromatin remodeling, V(D)J recombination, DNA repair and genome stability. Proposed to be an universal biosensor for nucleic acids. Promotes host inflammatory response to sterile and infectious signals and is involved in the coordination and integration of innate and adaptive immune responses. In the cytoplasm functions as a sensor and/or chaperone for immunogenic nucleic acids implicating the activation of TLR9-mediated immune responses, and mediates autophagy.

1. Yang, H. et al. (2013) Journal of leukocyte biology 93, 865-73. PMID: 23446148

2. Li, G. et al. (2013) Frontiers in immunology 4, 68. PMID: 23519706

3. Li, G. et al. (2013) Seminars in cancer biology 23, 380-90. PMID: 23994764

4. Lee, SA. et al. (2014) Yonsei medical journal 55, 1165-76. PMID: 25048472

5. Mitola, S. et al. (2006) Journal of immunology (Baltimore, Md. : 1950) 176, 12-5. PMID: 16365390

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Mouse HMGB1 Antibody (DPH1.1)
Western blot

Western blot analysis was performed using anti-HMGB1 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human HMGB1 (Catalog No: HY200011)
Lane 2: negative control

Reférences

Fiche technique

Anti-Mouse HMGB1 Recombinant Antibody (DPH1.1).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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