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Anti-Mouse HSPB1/HSP27 Polyclonal Antibody (MY322014)

Anti-Mouse HSPB1/HSP27 Polyclonal Antibody
Anti-Mouse HSPB1/HSP27 Polyclonal Antibody
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概要
カタログ番号MY322014
説明
Anti-Mouse HSPB1/HSP27 Polyclonal Antibody (MY322014) is a rabbit polyclonal antibody detecting HspB1 in ELISA, IHC, WB. Suitable for Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
種反応性Mouse, Rat
アプリケーションELISA, IHC, WB
宿主種Rabbit
クローン性Polyclonal
アイソタイプIgG
免疫原 E. coli - derived recombinant Mouse HSPB1/HSP27 (Ala76-Pro174).
ターゲット Heat shock protein beta-1, HspB1, Growth-related 25 kDa protein, Heat shock 25 kDa protein, HSP 25, Heat shock 27 kDa protein, HSP 27, p25, Hspb1, Hsp25, Hsp27
精製 Purified by antigen affinity column.
アクセッション番号 P14602
形態 Liquid
保存バッファー 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報
アプリケーション 希釈
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
安定性と保存 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Heat shock protein beta-1 (HSPB1/HSP27) is a ~23 kDa protein. Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state. Plays a role in stress resistance and actin organization. Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins.

1. Huang, L. et al. (2007) Genesis (New York, N.Y. : 2000) 45, 487-501. PMID: 17661394
注意事項 For research use only.
イメージ
  • Anti-Mouse HSPB1/HSP27 Polyclonal Antibody

    Western blot

    Lane 1: MCF-7 cell lysate
    Lane 2: Mouse heart lysate
    Lane 3: Rat heart lysate
    Lane 4: Mouse skeletal muscle lysate

参考文献
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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