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Обзор
Каталожный номерMV546014
Описание
Anti-Mouse KISS1R Polyclonal Antibody (MV546014) is a rabbit polyclonal antibody detecting KiSS-1 receptor; KiSS-1R; G-protein coupled receptor 54; G-protein coupled receptor OT7T175; mOT7T175; Kisspeptins receptor; Metastin receptor; Kiss1r; Gpr54 in ELISA, IHC, WB. Suitable for Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Реактивность видовMouse
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant mouse KISS1R (Ala2-Trp43&Leu102-Lys116&His181-Ala203&Val284-Lys305).
Цель KiSS-1 receptor; KiSS-1R; G-protein coupled receptor 54; G-protein coupled receptor OT7T175; mOT7T175; Kisspeptins receptor; Metastin receptor; Kiss1r; Gpr54
Уровень эндотоксинов Please contact with the lab for this information.
Очистка Purified by antigen affinity column.
Номер доступа Q91V45
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

KiSS-1 receptor (KISS1R) is a ~43 kDa protein. Receptor for kisspeptins (kisspeptin-10, kisspeptin-13, kisspeptin-14 and metastin/kisspeptin-54). The hypothalamic KISS1/KISS1R signaling system plays a central role in the regulation of the hypothalamic-pituitary-gonadal reproductive axis by modulating the secretion of gonadotropin-releasing hormone (GnRH) from GnRH neurons. In these neurons, kisspeptin binding to its receptor activates G(q)-dependent signaling, leading to phospholipase C (PLC) activation, and hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2). The subsequent rise in intracellular calcium levels results in the inhibition of inward rectifier potassium channels and activation of TRPC-like cation channels, leading to GnRH neurons depolarization and stimulation. In addition to this pathway, kisspeptin also triggers G(q)-independent signaling via beta-arrestin, leading to MAPK cascade activation and ERK1/ERK2 phosphorylation.

1. Stafford, LJ. et al. (2002) Cancer research 62, 5399-404. PMID: 12359743
2. Seminara, SB. et al. (2003) The New England journal of medicine 349, 1614-27. PMID: 14573733
3. Funes, S. et al. (2003) Biochemical and biophysical research communications 312, 1357-63. PMID: 14652023
4. Messager, S. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 1761-6. PMID: 15665093
5. Zhang, C. et al. (2008) The Journal of neuroscience : the official journal of the Society for Neuroscience 28, 4423-34. PMID: 18434521
6. Liu, X. et al. (2008) Endocrinology 149, 4605-14. PMID: 18483150
7. Ahow, M. et al. (2014) Endocrinology 155, 4433-46. PMID: 25147978
8. Li, Z. et al. (2024) Nature communications 15, 1300. PMID: 38346942
Примечание For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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