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Anti-Mouse Galectin-3 Polyclonal Antibody (MB167014)

Anti-Mouse Galectin-3 Polyclonal Antibody
Anti-Mouse Galectin-3 Polyclonal Antibody
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概要
カタログ番号MB167014
種反応性Mouse
アプリケーションELISA, IHC, WB
宿主種Rabbit
クローン性Polyclonal
アイソタイプIgG
免疫原 E. coli - derived recombinant Mouse LGALS3/Galectin-3 (Met1-Ile264).
ターゲット Galectin-3 (Gal-3) (35 kDa lectin) (Carbohydrate-binding protein 35) (CBP 35) (Galactose-specific lectin 3) (IgE-binding protein) (L-34 galactoside-binding lectin) (Laminin-binding protein) (Lectin L-29) (Mac-2 antigen)
精製 Purified by antigen affinity column.
アクセッション番号 P16110
形態 Liquid
保存バッファー 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報
アプリケーション 希釈
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
安定性と保存 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Galectin-3 (Gal-3) is a ~27 kDa protein. Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.

1. Fukushi, J. et al. (2004) Molecular biology of the cell 15, 3580-90. PMID: 15181153
注意事項 For research use only.
イメージ
  • Anti-Mouse Galectin-3 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-LGALS3 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: MCF-7 cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: Caco2 cell lysate

参考文献
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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