中文
English
한국어
日本語
Español
Français
Русский
| カタログ番号 | MV568014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Mouse MLKL Polyclonal Antibody (MV568014) is a rabbit polyclonal antibody detecting Mixed lineage kinase domain-like protein; Mlkl in ELISA, IHC, WB. Suitable for Mouse.
Highlights
|
||||||||
| 種反応性 | Mouse | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant mouse MLKL (Asp180-Val472). | ||||||||
| ターゲット | Mixed lineage kinase domain-like protein; Mlkl | ||||||||
| エンドトキシンレベル | Please contact with the lab for this information. | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | Q9D2Y4 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
||||||||
| 製品使用情報 |
| ||||||||
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Mixed lineage kinase domain-like protein (MLKL) is a ~54 kDa protein. Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Does not have protein kinase activity. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. In addition to TNF-induced necroptosis, necroptosis can also take place in the nucleus in response to orthomyxoviruses infection: following ZBP1 activation, which senses double-stranded Z-RNA structures, nuclear RIPK3 catalyzes phosphorylation and activation of MLKL, promoting disruption of the nuclear envelope and leakage of cellular DNA into the cytosol. Binds to highly phosphorylated inositol phosphates such as inositolhexakisphosphate (InsP6) which is essential for its necroptotic function. 1. Wu, J. et al. (2013) Cell research 23, 994-1006. PMID: 23835476 2. Murphy, JM. et al. (2013) Immunity 39, 443-53. PMID: 24012422 3. Kaiser, WJ. et al. (2013) The Journal of biological chemistry 288, 31268-79. PMID: 24019532 4. Nogusa, S. et al. (2016) Cell host & microbe 20, 13-24. PMID: 27321907 5. Zhang, T. et al. (2020) Cell 180, 1115-1129.e13. PMID: 32200799 6. Jiao, H. et al. (2020) Nature 580, 391-395. PMID: 32296175 | ||||||||
| 注意事項 | For research use only |
お問い合わせ
+86-027-65523339
中国武漢市深敦寺路666号C棟、武漢、430206
