

| Каталожный номер | MV568014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Описание |
Anti-Mouse MLKL Polyclonal Antibody (MV568014) is a rabbit polyclonal antibody detecting Mixed lineage kinase domain-like protein; Mlkl in ELISA, IHC, WB. Suitable for Mouse.
Highlights
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| Реактивность видов | Mouse | ||||||||
| Применения | ELISA, IHC, WB | ||||||||
| Хозяинский вид | Rabbit | ||||||||
| Клоональность | Polyclonal | ||||||||
| Изотип | IgG | ||||||||
| Иммуноген | E. coli - derived recombinant mouse MLKL (Asp180-Val472). | ||||||||
| Цель | Mixed lineage kinase domain-like protein; Mlkl | ||||||||
| Уровень эндотоксинов | Please contact with the lab for this information. | ||||||||
| Очистка | Purified by antigen affinity column. | ||||||||
| Номер доступа | Q9D2Y4 | ||||||||
| Форма | Liquid | ||||||||
| Буфер хранения | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии. |
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| Информация об использовании продукта |
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| Устойчивость и хранение | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| Фон | Mixed lineage kinase domain-like protein (MLKL) is a ~54 kDa protein. Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Does not have protein kinase activity. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. In addition to TNF-induced necroptosis, necroptosis can also take place in the nucleus in response to orthomyxoviruses infection: following ZBP1 activation, which senses double-stranded Z-RNA structures, nuclear RIPK3 catalyzes phosphorylation and activation of MLKL, promoting disruption of the nuclear envelope and leakage of cellular DNA into the cytosol. Binds to highly phosphorylated inositol phosphates such as inositolhexakisphosphate (InsP6) which is essential for its necroptotic function. 1. Wu, J. et al. (2013) Cell research 23, 994-1006. PMID: 23835476 2. Murphy, JM. et al. (2013) Immunity 39, 443-53. PMID: 24012422 3. Kaiser, WJ. et al. (2013) The Journal of biological chemistry 288, 31268-79. PMID: 24019532 4. Nogusa, S. et al. (2016) Cell host & microbe 20, 13-24. PMID: 27321907 5. Zhang, T. et al. (2020) Cell 180, 1115-1129.e13. PMID: 32200799 6. Jiao, H. et al. (2020) Nature 580, 391-395. PMID: 32296175 | ||||||||
| Примечание | For research use only |
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