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Anti-Mouse PM20D1 Polyclonal Antibody (MD457014)

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Anti-Mouse PM20D1 Polyclonal Antibody
Anti-Mouse PM20D1 Polyclonal Antibody
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  • 100ug
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Vista general
Número de catálogoMD457014
Descripción
Anti-Mouse PM20D1 Polyclonal Antibody (MD457014) is a rabbit polyclonal antibody detecting PM20D1. Suitable for Mouse.
Reactividad de especiesMouse
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Mouse PM20D1 (Lys233-Leu503).
Objetivo EC:3.5.1.114, EC:3.5.1.14, N-fatty-acyl-amino acid synthase/hydrolase PM20D1, PM20D1, Peptidase M20 domain-containing protein 1
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso Q8C165
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Fondo

N-fatty-acyl-amino acid synthase/hydrolase PM20D1 is a ~55 kDa protein. Secreted enzyme that regulates the endogenous N-fatty acyl amino acid (NAAs) tissue and circulating levels by functioning as a bidirectional NAA synthase/hydrolase. It condenses free fatty acids and free amino acids to generate NAAs and bidirectionally catalyzes the reverse hydrolysis reaction (Probable). Some of these NAAs stimulate oxidative metabolism via mitochondrial uncoupling, increasing energy expenditure in a UPC1-independent manner. Thereby, this secreted protein may indirectly regulate whole body energy expenditure. PM20D1 circulates in tight association with both low- and high-density (LDL and HDL,respectively) lipoprotein particles.

1. Long, JZ. et al. (2016) Cell 166, 424-435. PMID: 27374330
2. Lin, H. et al. (2018) Journal of medicinal chemistry 61, 3224-3230. PMID: 29533650
3. Long, JZ. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, E6937-E6945. PMID: 29967167
4. Kim, JT. et al. (2020) eLife 9. PMID: 32271712
5. Kim, JT. et al. (2020) Cell chemical biology 27, 1130-1139.e4. PMID: 32402239
Nota For research use only
Imágenes
  • Anti-Mouse PM20D1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-PM20D1 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Mouse liver lysate

Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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