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Anti-Mouse PM20D1 Polyclonal Antibody (MD457014)

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Anti-Mouse PM20D1 Polyclonal Antibody
Anti-Mouse PM20D1 Polyclonal Antibody
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  • 50ug
  • 100ug
Nombre :
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Aperçu
Numéro de catalogueMD457014
Description
Anti-Mouse PM20D1 Polyclonal Antibody (MD457014) is a rabbit polyclonal antibody detecting PM20D1. Suitable for Mouse.
Reactivité des espècesMouse
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Mouse PM20D1 (Lys233-Leu503).
Cible EC:3.5.1.114, EC:3.5.1.14, N-fatty-acyl-amino acid synthase/hydrolase PM20D1, PM20D1, Peptidase M20 domain-containing protein 1
Niveau d'endotoxines Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Numéro d'accès Q8C165
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Contexte

N-fatty-acyl-amino acid synthase/hydrolase PM20D1 is a ~55 kDa protein. Secreted enzyme that regulates the endogenous N-fatty acyl amino acid (NAAs) tissue and circulating levels by functioning as a bidirectional NAA synthase/hydrolase. It condenses free fatty acids and free amino acids to generate NAAs and bidirectionally catalyzes the reverse hydrolysis reaction (Probable). Some of these NAAs stimulate oxidative metabolism via mitochondrial uncoupling, increasing energy expenditure in a UPC1-independent manner. Thereby, this secreted protein may indirectly regulate whole body energy expenditure. PM20D1 circulates in tight association with both low- and high-density (LDL and HDL,respectively) lipoprotein particles.

1. Long, JZ. et al. (2016) Cell 166, 424-435. PMID: 27374330
2. Lin, H. et al. (2018) Journal of medicinal chemistry 61, 3224-3230. PMID: 29533650
3. Long, JZ. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, E6937-E6945. PMID: 29967167
4. Kim, JT. et al. (2020) eLife 9. PMID: 32271712
5. Kim, JT. et al. (2020) Cell chemical biology 27, 1130-1139.e4. PMID: 32402239
Note For research use only
Images
  • Anti-Mouse PM20D1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-PM20D1 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Mouse liver lysate

Reférences
Fiche technique
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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