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Anti-Mouse PM20D1 Polyclonal Antibody
Anti-Mouse PM20D1 Polyclonal Antibody
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Спецификация:
  • 50ug
  • 100ug
Количество:
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Обзор
Каталожный номерMD457014
Описание
Anti-Mouse PM20D1 Polyclonal Antibody (MD457014) is a rabbit polyclonal antibody detecting PM20D1. Suitable for Mouse.
Реактивность видовMouse
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Mouse PM20D1 (Lys233-Leu503).
Цель EC:3.5.1.114, EC:3.5.1.14, N-fatty-acyl-amino acid synthase/hydrolase PM20D1, PM20D1, Peptidase M20 domain-containing protein 1
Уровень эндотоксинов Please contact with the lab for this information.
Очистка Purified by antigen affinity column.
Номер доступа Q8C165
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

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Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Фон

N-fatty-acyl-amino acid synthase/hydrolase PM20D1 is a ~55 kDa protein. Secreted enzyme that regulates the endogenous N-fatty acyl amino acid (NAAs) tissue and circulating levels by functioning as a bidirectional NAA synthase/hydrolase. It condenses free fatty acids and free amino acids to generate NAAs and bidirectionally catalyzes the reverse hydrolysis reaction (Probable). Some of these NAAs stimulate oxidative metabolism via mitochondrial uncoupling, increasing energy expenditure in a UPC1-independent manner. Thereby, this secreted protein may indirectly regulate whole body energy expenditure. PM20D1 circulates in tight association with both low- and high-density (LDL and HDL,respectively) lipoprotein particles.

1. Long, JZ. et al. (2016) Cell 166, 424-435. PMID: 27374330
2. Lin, H. et al. (2018) Journal of medicinal chemistry 61, 3224-3230. PMID: 29533650
3. Long, JZ. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, E6937-E6945. PMID: 29967167
4. Kim, JT. et al. (2020) eLife 9. PMID: 32271712
5. Kim, JT. et al. (2020) Cell chemical biology 27, 1130-1139.e4. PMID: 32402239
Примечание For research use only
Изображения
  • Anti-Mouse PM20D1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-PM20D1 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Mouse liver lysate

Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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