Anti-Mouse PPIA Polyclonal Antibody (MX128014)

Name: Anti-Mouse PPIA Polyclonal Antibody
Brand: abinScience
SKU: MX128014
Price: 168 USD
Availability: InStock

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Prix(USD) : --

Spécification :

50ug
100ug

Nombre :

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Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

MX128014

Description

Anti-Mouse PPIA Polyclonal Antibody (MX128014) is a rabbit polyclonal antibody detecting Ppia. Suitable for Rat, Mouse, and Human.

Highlights

●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Mouse PPIA (Met1-Leu164).

Cible

Cyclophilin A, Cyclosporin A-binding protein, EC:5.2.1.8, PPIase A, Peptidyl-prolyl cis-trans isomerase A, Peptidyl-prolyl cis-trans isomerase A, N-terminally processed, Ppia, Rotamase A, SP18

Niveau d'endotoxines

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Numéro d'accès

P17742

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Contexte

Peptidyl-prolyl cis-trans isomerase A (PPIA) is a ~17 kDa protein. Catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Exerts a strong chemotactic effect on leukocytes partly through activation of one of its membrane receptors BSG/CD147, initiating a signaling cascade that culminates in MAPK/ERK activation. Activates endothelial cells (ECs) in a pro-inflammatory manner by stimulating activation of NF-kappa-B and ERK, JNK and p38 MAP-kinases and by inducing expression of adhesion molecules including SELE and VCAM1. Induces apoptosis in ECs by promoting the FOXO1-dependent expression of CCL2 and BCL2L11 which are involved in EC chemotaxis and apoptosis. In response to oxidative stress, initiates proapoptotic and antiapoptotic signaling in ECs via activation of NF-kappa-B and AKT1 and up-regulation of antiapoptotic protein BCL2.

1. Wang, L. et al. (2014) Thrombosis and haemostasis 111, 873-82. PMID: 24429998

2. Lauranzano, E. et al. (2015) Brain : a journal of neurology 138, 974-91. PMID: 25678563

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-PPIA polyclonal antibody at 1ug/mL on various samples.
Lane 1: HepG2 cell lysate
Lane 2: NIH3T3 cell lysate
Lane 3: K562 cell lysate
Lane 4: Raji cell lysate
Lane 5: Jurkat cell lysate
Lane 6: PC-12 cell lysate
Lane 7: 293T cell lysate
Lane 8: Mouse brain lysate
Lane 9: Rat brain lysate

Reférences

Fiche technique

Anti-Mouse PPIA Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

Kits d'Essai

Anti-Mouse PPIA Polyclonal Antibody (MX128014)

Aperçu

Images

Reférences

Fiche technique

SDS