中文
English
한국어
日本語
Español
Français
Русский
| カタログ番号 | MC317014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Mouse RIPK1 Polyclonal Antibody (MC317014) is a rabbit polyclonal antibody detecting Ripk1 in ELISA, IHC, WB. Suitable for Mouse and Rat.
Highlights
|
||||||||
| 種反応性 | Mouse | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Mouse RIPK1 (Met1-Pro310). | ||||||||
| ターゲット | Receptor-interacting serine/threonine-protein kinase 1, 2.7.11.1, Cell death protein RIP, Receptor-interacting protein 1, RIP-1, Ripk1, Rinp, Rip | ||||||||
| エンドトキシンレベル | Please contact with the lab for this information. | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | Q60855 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
||||||||
| 製品使用情報 |
| ||||||||
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Receptor-interacting serine/threonine-protein kinase 1 (RIPK1) is a ~74 kDa protein. Serine-threonine kinase which is a key regulator of TNF-mediated apoptosis, necroptosis and inflammatory pathways. Exhibits kinase activity-dependent functions that regulate cell death and kinase-independent scaffold functions regulating inflammatory signaling and cell survival. Has kinase-independent scaffold functions: upon binding of TNF to TNFR1, RIPK1 is recruited to the TNF-R1 signaling complex (TNF-RSC also known as complex I) where it acts as a scaffold protein promoting cell survival, in part, by activating the canonical NF-kappa-B pathway. Kinase activity is essential to regulate necroptosis and apoptosis, two parallel forms of cell death: upon activation of its protein kinase activity, regulates assembly of two death-inducing complexes, namely complex IIa (RIPK1-FADD-CASP8), which drives apoptosis, and the complex IIb (RIPK1-RIPK3-MLKL), which drives necroptosis. RIPK1 is required to limit CASP8-dependent TNFR1-induced apoptosis. 1. Newton, K. et al. (2014) Science (New York, N.Y.) 343, 1357-60. PMID: 24557836 2. Rickard, JA. et al. (2014) Cell 157, 1175-88. PMID: 24813849 3. Dillon, CP. et al. (2014) Cell 157, 1189-202. PMID: 24813850 4. Lin, J. et al. (2016) Nature 540, 124-128. PMID: 27819681 5. Geng, J. et al. (2017) Nature communications 8, 359. PMID: 28842570 6. Newton, K. et al. (2019) Nature 574, 428-431. PMID: 31511692 7. Tao, P. et al. (2020) Nature 577, 109-114. PMID: 31827280 8. Lalaoui, N. et al. (2020) Nature 577, 103-108. PMID: 31827281 9. Muendlein, HI. et al. (2021) Nature communications 12, 86. PMID: 33397971 | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-RIPK1 polyclonal antibody at RIPK1 on various samples.
Lane 1: Rat heart lysate
+86-027-65523339
中国武漢市深敦寺路666号C棟、武漢、430206
